English Commentary Patch Pes 6

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English Commentary Patch Pes 6

 

May 5, 2019
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English commentary patch pes 6The invention relates to a test method for bone tissue.
The method comprises mixing collagen and a gel-forming agent and subjecting the mixture to a freeze-thawing cycle in the presence of CaCl2.
It is known to use collagen and gel-forming agents in the fabrication of artificial organs or biological implants (cf. German patent No. 1,173,664). The objective is to find a suitable substrate for the ingrowth of new bone tissue. Collagen-containing gels are known to have a low viscosity, and so the gels readily permeate the porous bodies of implants. The biological effect of the implanted collagen matrix is largely determined by its internal structure. The structural properties of the collagen are a function of its crosslinking with gel-forming agents. Among the agents used in this connection are calcium chloride, thioglycolic acid and tartaric acid. In the published literature there are reports on the chemical crosslinking of collagen with, for example, calcium chloride and thioglycolic acid (U.S. Pat. No. 3,637,636). A gel-forming agent is necessary in order to maintain the primary structure of the collagen.
If the collagen is to act as an osteogenetic carrier, the gel-forming agent must be chemically bound to the collagen.
European patent application No. 00 7654 discloses a process for the preparation of synthetic collagen matrices from collagen gel and a gel-forming agent, and for the implantation of these matrices in vivo. It has been demonstrated that the porous matrices that are produced in accordance with the invention have a good osteogenic potential.
The use of calcium chloride or thioglycolic acid, or both, is a prerequisite for the crosslinking of collagen. The disadvantage of the cited methods is that, for example, calcium chloride, which has a strong tendency to bind to protein molecules, can cause the destruction of collagen (formation of a rigid cement), which is undesirable in the intended application of the implanted material (

 

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